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stable cell line generation of gl261 mgmt  (Creative Biogene Inc)

 
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    Creative Biogene Inc stable cell line generation of gl261 mgmt
    Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in <t>GL261</t> cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.
    Stable Cell Line Generation Of Gl261 Mgmt, supplied by Creative Biogene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stable cell line generation of gl261 mgmt/product/Creative Biogene Inc
    Average 90 stars, based on 1 article reviews
    stable cell line generation of gl261 mgmt - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Novel Imidazotetrazine Evades Known Resistance Mechanisms and Is Effective against Temozolomide-Resistant Brain Cancer in Cell Culture"

    Article Title: Novel Imidazotetrazine Evades Known Resistance Mechanisms and Is Effective against Temozolomide-Resistant Brain Cancer in Cell Culture

    Journal: ACS chemical biology

    doi: 10.1021/acschembio.2c00022

    Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in GL261 cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.
    Figure Legend Snippet: Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in GL261 cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.

    Techniques Used: Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Quantitation Assay, Control, Western Blot



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    stable cell line generation of gl261 mgmt  (Creative Biogene Inc)
    90
    Creative Biogene Inc stable cell line generation of gl261 mgmt
    Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in <t>GL261</t> cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.
    Stable Cell Line Generation Of Gl261 Mgmt, supplied by Creative Biogene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stable cell line generation of gl261 mgmt/product/Creative Biogene Inc
    Average 90 stars, based on 1 article reviews
    stable cell line generation of gl261 mgmt - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in GL261 cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.

    Journal: ACS chemical biology

    Article Title: Novel Imidazotetrazine Evades Known Resistance Mechanisms and Is Effective against Temozolomide-Resistant Brain Cancer in Cell Culture

    doi: 10.1021/acschembio.2c00022

    Figure Lengend Snippet: Investigation of the mechanism of action and efficacy of CPZ. (A) Detection of O6-methyl-2′-deoxyguanosine or O6-propargyl-2′-deoxyguanosine in GL261 cells after treatment with TMZ or CPZ, respectively. GL261 cells were treated with the indicated concentration of compound for 8 h before they were harvested and genomic DNA was extracted. DNA (10 μg) was hydrolyzed and submitted to LC–MS/MS for quantitation. Both O6-Me-dG and O6-proparyl-dG were below the limit of detection in the DMSO control and CPZ samples. Error is SEM, n ≥ 3.(B) Cytotoxicity of TMZ (left) or CPZ (right) in T98G cells pretreated (3 h) with MGMT inhibitor O6BG. Error is SEM, n ≥ 3. (C) Western blot of GL261, GL261 MGMT+, and T98G (10 μg protein loading, 1:1000 anti-MGMT antibody). (D) 7 day dose–response curves of TMZ and CPZ in GL261 cells. Error is SEM, n ≥ 3. (E) 7 day dose–response curves of TMZ and CPZ in GL261 MGMT+ cells. Error is SEM, n ≥ 3.

    Article Snippet: Stable cell line generation of GL261 MGMT+ was purchased from Creative Biogene.

    Techniques: Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Quantitation Assay, Control, Western Blot